Method and apparatus for measuring lipid peroxide
AssigneeTohoku Electronic Industrial Co., Ltd.
InventorsTeruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto, Akio Saeki
Life of this patent
Estimating the term…
Abstract
A method and apparatus for measuring a lipid peroxide, in which a sample containing lipids is subjected to a liquid chromatography to separate the lipids into lipid classes. The lipid classes are brought into contact with a luminescent reagent which specifically reacts with a lipid hydroperoxide contained in the lipid classes to generate a light in an amount corresponding to a content of the hydroperoxide. The light is optically detected by a photodetector.
Claims9
A method of detecting a lipid hydroperoxide, comprising:
- subjecting a sample containing lipids to a liquid chromatography to separate the lipids into lipid classes;
- bringing the lipid classes into contact with a luminescent reagent which specifically reacts with lipid hydroperoxide contained in the lipid classes to generate a light in an amount corresponding to a content of the hydroperoxide;
- and
- optically detecting said light by photodetecting means.
A method of detecting a lipid hydroperoxide, comprising:
- a. subjecting a sample containing lipids to a liquid chromatography to separate the lipids into lipid classes;
- b. bringing the lipid classes into contact with a luminescent reagent which specifically reacts with a lipid hydroperoxide contain.RTM.in the lipid classes to generate a light in an amount corresponding to a content of the hydroperoxide;
- c. optically detecting said light by photodetecting means;
- d. said luminescent reagent including a catalyst that acts on the hydroperoxide to produce active oxygen species, and a luminescent substance which reacts with the active oxygen species to emit light;
- and
- e. said catalyst including a material selected from a group consisting of a heme protein, heme peptide, and heme enzyme.
Dependencies are inferred from the claim text.