Method for determining the existence and/or the monitoring of a pathological condition in a mammal and a test kit therefor
AssigneeUniversity of Medicine and Dentistry of New Jersey
InventorsCharles R. Spillert, William A. Suval, Eric J. Lazaro
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Abstract
There is disclosed a method of testing, and a kit therefor, a cellular hematologic fluid derived from a mammal to determine the existence in the mammal of a pathological state or condition wherein an immunomodulator is admixed with the cellular hematologic fluid of the mammal and a reaction parameter determined and compared with known reaction parameters of cellular hematologic fluids of mammals of known healthy states to like immunomodulator. In a preferred embodiment of the present invention, the reaction parameter is a clotting parameter as determined as fibrin levels.
Claims14
A prepackaged diagnostic kit for analyzing the blood of a mammal to determine the presence or development of pathology suspected of causing abnormalities in the immune response, system and/or the blood coagulated of the mammal comprising:
- A. at least one first container having a predetermined anticoagulant disposed therein, for receiving whole blood and rendering the same anticoagulated;
- B. at least one second container serving for the preparation of a control sample when an aliquot portion of the anticoagulated blood from said first container is introduced therein;
- C. at least one third container having a predetermined quantity of a modulator for reception of a further aliquot of said anticoagulated blood from said first container;
- D. means for initiating the clotting of said control sample and said activated sample to facilitate a subsequent measurement of the reaction parameters of each of said samples;
- and
- E. a diagnostic protocol for the determination of the pathological state of said mammal by the analysis of said control and said activated blood sample by the comparison of the respective reaction parameters thereof.
A method for analyzing the blood of a mammal to determine the presence or development of pathology suspected of causing abnormalities in the immune response system and/or the blood coagulation of the mammal consisting essentially of:
- A. preparing a quantity of anticoagulated whole blood from a sample of whole blood taken from a mammal;
- B. taking an aliquot portion of said anticoagulated blood and introduing said aliquot portion into a first container and thereby preparing a control sample;
- C. taking a further aliquot portion of said anticoagulated blood and introducing said further aliquot portion into a second container having therein a modulator and thereby preparing an activated sample;
- D. incubating said control sample and said activated sample at a predetermined suitable incubation temperature from about 1 to about 4 hours;
- E. initiating clotting activity and measuring a reaction parameter for each of said control sample and said activated sample;
- and
- F. identifying the presence of pathology by comparing the reaction parameters measured in Step E. with similar reaction parameters measured from a mammal in a healthy state.
Dependencies are inferred from the claim text.