US 4,900,676 A · Method of producing herbicide resistant plant varieties and plants produced thereby

Abstract

A method of introducing cytoplasmically inherited traits from a donor plant into a receptor plant of the Solanaceae family has been invented. A method is provided for producing Solanum tuberosum plants which have the inherited trait. Protoplasts from the donor and receptor plants are prepared. The donor protoplasts are treated under suitable conditions to prevent extensive nuclear divisions. The treated donor protoplasts and receptor protoplasts are then contacted under suitable conditions permitting the fusion of the protoplasts. Plantlets are regenerated from the fused protoplasts, and plantlets which contain the donor cytoplasmic traits are selected and rooted. Plantlets which contain recpetor cytoplasmic traits are discarded.

Claims10

Dependent claims

A method of producing triazine-resistant Solanum tuberosum cv. Mirka plants which comprises:

(a) vegetatively propagating calli or Solanum tuberosum cv. Mirka Tri-R C-165 (ATCC Accession No. 40164) in a first growth medium comprising the modified Murashige and Skoog basal medium of Table 1 to which has been added about 0.1 mg/l gibberellic acid, about 0.2 mg/l abscissic acid, about 10 mg/l zeatin (trans-isomer) and about 10 mg/l kinetin under suitable conditions to generate plantlets having shoots and roots;
and
(b) transferring the plantlets having a sufficient root system to a suitable second growth medium, wherein the rooted plantlets are propagated under suitable conditions to produce mature triazine-resistant Solanum tuberosum cv. Mirka plants.

Dependencies are inferred from the claim text.

Method of producing herbicide resistant plant varieties and plants produced thereby

Life of this patent

Method of producing herbicide resistant plant varieties and plants produced thereby

Life of this patent

Abstract

Claims10