Method for preservation of nitrile hydration activity
AssigneeNitto Kagaku Kogyo Kabushiki Kaisha
InventorsHideaki Yamada, Kanehiko Enomoto, Koitchiro Ryuno, Hitoshi Shimizu
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Abstract
Nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas or Pseudomonas microorganism cells per se or in the immobilized state, all having nitrile hydration activity can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a suspension or solution of the nitrile hydratase, or the microorganism cells per se or in the immobilized state.
Claims5
A method for preservation of the nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas, of cells of microorganisms of said genus Pseudomonas, or of immobilized forms thereof, all having nitrile hydration activity, said method comprising admixing as a stabilizer at least one compound selected from the group consisting of nitriles, amides, and organic acids and salts thereof with a suspension or solution of said nitrile hydratase, of said microorganism cells, or of said immobilized forms thereof, and the concentration of nitrile, amide and organic acid or the salt thereof in the suspension or solution being in the range of from 0.01 to 50 g/l.
Dependencies are inferred from the claim text.