Reagent for detection and measurement of acetaldehyde-protein condensates in a fluid, together with its preparation and method of use
AssigneeAlcoholism and Drug Addition Research Foundation
InventorsYedy Israel, Ruth Arnon
Life of this patent
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Abstract
A method is provided for detecting and measuring acetaldehyde-protein condensates. An antibody is produced to an antigen, the antibody being cross-reactive with acetaldehyde-protein condensates, these condensates including protein moieties corresponding to or different from the protein moiety of the antigen. Detection and measurement of acetaldehyde-protein condensates may be performed by reacting the antibody with the acetaldehyde-protein condensate to form a complex and measuring the complex.
Claims7
A method for the detection and quantification of an acetaldehyde-protein condensate comprising reacting said condensate with an antibody specific to an acetaldehyde-protein condensate antigen which antibody is characterized by being cross-reactive with a reactant selected from the group consisting of:
- an acetaldehyde-first protein condensate;
- an acetaldehyde-second protein condensate;
- and
- an acetaldehyde-modified protein, wherein said acetaldehyde-first protein condensate comprises a first protein moiety corresponding to the protein moiety of said antigen;
- and
- said acetaldehyde-second protein condensate comprises a second protein moiety different from the protein moiety of said antigen;
- and
- said acetaldehyde-modified protein corresponding to said protein moiety of said antigen, in order to form a complex;
- and
- measuring said complex, wherein the amount of said complex is a function of the concentration of the acetaldehyde-protein condensate present.
An antibody specific to an acetaldehyde-protein condensate antigen which antibody is characterized by being cross-reactive with a reactant selected from the group consisting of:
- an acetaldehyde-first protein condensate;
- an acetaldehyde-second protein condensate;
- and
- an acetaldehyde-modified protein;
- wherein
- said acetaldehyde-first protein condensate comprises a first protein moiety corresponding to the protein moiety of said antigen;
- said acetaldehyde-second protein condensate comprises a second protein moiety different from said protein moiety of said antigen;
- and
- said acetaldehyde-modified protein corresponding to said protein moiety of said antigen.
An antibody which recognises acetaldehyde-containing determinants of acetaldehyde-protein condensates.
A substantially immunologically pure antigen which stimulates the production of an antibody cross-reactive with acetaldehyde-protein condensates formed at acetaldehyde concentrations of substantially 100 .mu.M or less.
A substantially immunologically pure antigen which comprises an acetaldehyde-protein condensate produced by the condensation of a protein with acetaldehyde at a concentration within the range 150 to 300 mM.
A substantially immunologically pure antigen which comprises an acetaldehyde-protein condensate, said condensate including a hemoglobin constituent which comprises 50-98% acetaldehyde-conjugated hemoglobin.
Dependencies are inferred from the claim text.