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AssigneesInstitut Pasteur of Paris, Centre National de la Recherche Scientifique (CNRS)
InventorsJean-Luc Guesdon, Stratis Avrameas, Jean-Claude Mazie
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This method for immunological determination of amines in biological media consists of: (a) reacting a quinone (in particular, p-benzoquinone) with the amine (for example histamine) present in the test sample; (b) introducing into the medium a monoclonal antibody which specifically recognizes the amine-quinone reaction product formed; and (c) determining one of the forms of the monoclonal antibody present. When the quinone is used in the immobilized state, the monoclonal antibody present is determined in its complexed form with the amine-quinone reaction product (direct type method); when the quinone is used in the free state, the monoclonal antibody is determined in its free form (enzymoimmunometric method or competitive immunoenzymatic method).
A method for immunological determination of amines in biological media, wherein:
The method as claimed in claim 7, wherein a peroxidase is used as the enzyme.
The method as claimed in claim 9, wherein the amine in the sample is histamine.
The monoclonal antibodies specific for the product resulting from the reaction of a quinone with a primary or secondary amine.
The monoclonal antibodies as claimed in claim 12, which are specific for the product resulting from the reaction of a primary amine with p-benzoquinone.
The monoclonal antibodies as claimed in claim 14, having the characteristics of those secreted by the hybridoma cell line which have been deposited with the CNCM under the no. I-421.
Hybridomas having the characteristics of those belonging to the cell line which have been deposited with the CNCM under the no. I-421.
Dependencies are inferred from the claim text.