Centrifugal fast chromatograph
AssigneeLarge Scale Biology Corporation
InventorNorman G. Anderson
Life of this patent
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Abstract
A centrifugal fast chromatograph is disclosed. The apparatus includes a rotor, a rotor drive, a plurality of chromatographic columns mounted on the rotor, a transfer disk mounted on the rotor, photodetecting apparatus, a gradient maker and a microprocessor control system. The microprocessor controls and monitors the entire chromatography process. The centrifugal fast chromatograph permits the performance of multiple, rapid, and simultaneous separations.
Claims11
A method of simultaneously conducting gradient-elution of a plurality of solutions using a centrifugal fast chromatograph having a valve system for reversing flow through columns while maintaining centrifugally-generated pressure and flow comprising the steps of:
- placing a sample in a transfer disk of a chromatograph rotor;
- placing gradient solutions in a gradient maker;
- transferring a gradient from said gradient maker through gradient feed lines, distributor rings, and feed lines, and into chromatographic columns;
- eluting the samples by the gradient and by rotating said chromatographic columns;
- monitoring the separated solutions with a photodetector and a light source which detect the state of the solutions through an optical flow cell disposed in each said chromatographic column;
- and
- simultaneously controlling, monitoring and recording all aspects of the chromatographic process for said chromatographic columns.
A method of simultaneously conducting elution of a plurality of sample solutions using a centrifugal chromatograph having a plurality of rotatable chromatographic columns and a valve system for reversing flow through the columns while maintaining centrifugally-generated pressure and flow comprising the steps of:
- selectively placing a sample in flow communication with either end of a chromatographic column;
- selectively placing solutions in flow communication with said chromatographic columns for eluting the sample from said selected end;
- eluting the sample with the gradient by rotating said chromatographic columns to produce separated solutions in the columns.
Dependencies are inferred from the claim text.