Electrophoretic method
AssigneeFuji Photo Film Co., Ltd.
InventorsYoshihiko Makino, Masashi Ogawa
Life of this patent
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Abstract
An electrophoretic method comprises the steps of positioning a sample, which contains nucleic acid fragments having different numbers of bases, in an electrophoresis medium composed of an electrophoresis gel medium containing an aqueous poly(meth)acrylamide gel prepared by cross-linking polymerization of a (meth)acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent (or modifier), and applying pulsed electric fields to the electrophoresis medium in two directions, thereby to move the nucleic acid fragments in the presence of molecular sieve effects and to separate the nucleic acid fragments.
Claims9
An electrophoretic method comprising the steps of:
- (i) positioning a sample, which contains nucleic acid fragments having different numbers of bases, in an electrophoresis medium composed of an electrophoresis gel medium containing an aqueous poly(meth)acrylamide gel prepared by the cross-linking polymerization of a (meth)acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent, and
- (ii) applying pulsed electric fields to said electrophoresis medium in two directions, the electric field strength in each direction being from about 10 V/cm to 150 V/cm, and the duration of each pulse being from about 0.1 msec to 100 msec, to move thereby said nucleic acid fragments in the presence of a molecular sieve effect and to separate said nucleic acid fragments.
Dependencies are inferred from the claim text.