Enrichment and concentration of proteins by ultrafiltration
AssigneeAbbott Laboratories
InventorsChristopher T. Cordle, Larry G. Criswell, Ronald L. Thomas
Life of this patent
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Abstract
Disclosed are ultrafiltration processes for the enrichment and concentration of selected proteins from protein conaining fluids. Illustratively, IgG is concentrated from whey by means of multiple ultrafiltration "passes", using a metallic oxide ultrafiltration membrane, which are characterized by differing pH's.
Claims40
A process for concentrating a protein from a fluid and enriching the protein with respect to concentrations of proteins having different sizes and/or isoelectric points, said process comprising:
- (a) introducing a fluid to a first metallic oxide ultrafiltration membrane, at a pH which is below the isoelectric point of a selected protein, whereby a retentate containing the selected protein is produced.
- (b) subjecting said retentate to a second ultrafiltration at a pH above the isoelectric point of the selected protein, whereby a permeate containing the selected protein is produced;
- and
- (c) collecting the permeate containing the selected protein.
A process for concentrating a protein from a fluid and enriching the protein with respect to concentrations of proteins having different sizes and/or isoelectric points, said process comprising:
- (a) introducing a fluid to a first metallic oxide ultrafiltration membrane, at a pH which is above the isoelectric point of a selected protein, whereby a permeate containing the selected protein is produced;
- (b) subjecting said permeate to a second ultrafiltration at a pH below the isoelectric point of the protein, whereby a retentate containing the selected protein is produced;
- and
- (c) collecting the retentate containing the selected protein.
A process for concentrating an immunoglobulin from a fluid and enriching the immunoglobulin with respect to concentrations of proteins having different sizes and/or isoelectric points, said process comprising:
- (a) introducing a fluid to a first metallic oxide ultrafiltration membrane, at a pH which is below the isoelectric point of an immunoglobulin, whereby a retentate containing the immunoglobulin is produced;
- (b) subjecting said retentate to a second ultrafiltration at a pH above the isoelectric point of the immunoglobulin, whereby a permeate containing the immunoglobulin is produced;
- and
- (c) collecting the permeate containing the immunoglobulin.
A process for concentrating an immunoglobulin from a fluid and enriching the immunoglobulin with respect to concentrations of proteins having different sizes and/or isoelectric points, said process comprising:
- (a) introducing a fluid to a first metallic oxide ultrafiltration membrane, at a pH which is above the isoelectric point of an immunoglobulin, whereby a permeate containing the immunoglobulin is produced;
- (b) subjecting said permeate to a second ultrafiltration at a pH below the isoelectric point of the immunoglobulin, whereby a retentate containing the immunoglobulin is produced;
- and
- (c) collecting the retentate containing the immunoglobulin.
A process for concentrating an immunoglobulin from whey and enriching the immunoglobulin with respect to concentrations of other proteins having a different size and isoelectric point, said process comprising:
- (a) introducing a whey feed to a first metallic oxide ultrafiltration membrane, wherein the metallic oxide is deposited on a rigid porous surface, at a pH of 5.0, which is below the isoelectric point of an immunoglobulin, whereby a first retentate containing the immunoglobulin is produced;
- (b) submitting said first retentate to an ultrafiltration diafiltration with water at constant volume, at the parameters of said first ultrafiltration, the volume of water used for the diafiltration being about one-fifth the initial protein solution volume;
- (c) subjecting said first retentate to a second ultrafiltration at the parameters of said first ultrafiltration except that the pH is equal to 7.0, whereby a permeate containing the immunoglobulin is produced;
- and
- (d) collecting the permeate containing the immunoglobulin.
Dependencies are inferred from the claim text.