# US 4,891,219 A · Method for immunization against and treatment of infection by ectoparasites and endoparasites

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- Number: US 4,891,219 A
- Document ID: US4891219A
- Status: Granted
- Kind: A
- Application number: 07/096,699
- Filed: 1987-09-14
- Granted: 1990-01-02
- Earliest claimed: 1986-03-14
- Assignees: Aphton Corporation
- Inventors: Stephen L. Karr, Jr.; Elizer Benjamini; Robert J. Scibienski; Stephen Grimes
- Web page: https://patentgrep.com/patent/US4891219A

## CPC

- A61K 39/0003: {Invertebrate antigens}
- A61K 39/44: Antibodies bound to carriers
- A61K 47/646: {the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines}
- A61K 2039/6081: {Albumin; Keyhole limpet haemocyanin [KLH]}
- Y10S 530/808: Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
- Y10S 530/81: Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S 530/811: Peptides or proteins is immobilized on, or in, an inorganic carrier
- Y10S 530/812: Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S 530/816: Attached to the carrier via a bridging agent
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

This invention relates to a method for the active or passive immunization of a vertebrate against ectoparasites and endoparasites, and a method of treating a vertebrate host infected by ectoparasites or endoparasites, comprising administering to the vertebrate an immunogen comprising one or more endocrine products of ectoparasites and endoparasites, coupled with an immunogenic carrier, or administering to the vertebrate, monoclonal antibodies capable of binding to the native form of the endocrine product.

## Claims

### Claim 1

A pharmaceutical composition for use in a vertebrate host as a vaccine or a medicament for immunizing against or treating for infection by an invertebrate ectoparasite or endoparasite comprising an immunogen that is capable of eliciting antibodies in the host that bind to the native form of juvenile hormone or beta-ecdysone and have a biological effect on the ectoparasite or endoparasite, wherein the immunogen comprises an endocrine product of the ectoparasite or endoparasite selected from the group consisting of juvenile hormone, beta-ecdysone or analogs thereof, conjugated to a protein selected from the group consisting of mino acids or chains of amino acids, straight chain hydrocarbons, aromatic derivatives, gallic acid, or polyvinyl alcohol carrier by means of a spacer molecule and a pharmaceutically acceptable carrier.

### Claim 2 (depends on 1)

A method of immunizing a vertebrate against or treating a vertebrate for infection by endoparasites or ectoparasites comprising administering to the vertebrate an immunologically effective amount of a composition of claim 1.

### Claim 3

A pharmaceutical composition for use in a vertebrate host as a vaccine or a medicament for immunizing against or treating for infection by an invertebrate ectoparasite or endoparasite comprising an immunogen that is capable of eliciting antibodies in the host that bind to the native form of juvenile hormone or beta-ecdysone and have a biological effect on the ectoparasite or endoparasite, wherein the immunogen comprises an endocrine product of the ectoparasite or endoparasite selected from the group consisting of juvenile hormone, beta-ecdysone or analogs thereof, conjugated to keyhole limpet hemocyanin, and a pharmaceutically acceptable carrier.

### Claim 4

A pharmaceutical composition for use in a vertebrate host as a vaccine or a medicament for immunizing against or treating for infection by an invertebrate ectoparasite or endoparasite comprising an immunogen that is capable of eliciting antibodies in the host that bind to the native form of juvenile hormone or beta-ecdysone and have a biological effect on the ectoparasite or endoparasite, wherein the immunogen comprises an endocrine product of the ectoparasite or endoparasite selected from the group consisting of juvenile hormone, beta-ecdysone or analogs thereof, conjugated to diphtheria toxoid, and a pharmaceutically acceptable carrier.

### Claim 5

A pharmaceutical composition for use in a vertebrate host as a vaccine or a medicament for immunizing against or treating for infection by an invertebrate ectoparasite or endoparasite comprising an immunogen that is capable of eliciting antibodies in the host that bind to the native form of juvenile hormone and have a biological effect on the ectoparasite or endoparasite, wherein the immunogen comprises juvenile hormone, or analogs thereof, conjugated to a protein carrier by means of a spacer molecule of gallic acid, and a pharmaceutically acceptable carrier.

### Claim 6

A method of immunizing a vertebrate against or treating a vertebrate for infection by an ectoparasite or endoparasite comprising administering to the vertebrate an immunologically effective amount of a first immunogen comprising juvenile hormone or an analog thereof conjugated to a protein carrier and an immunologically effective amount of a second immunogen comprising beta-ecdysone or an analog thereof conjugated to a protein carrier.

### Claim 7

A pharmaceutical composition for use in a host vertebrate as a vaccine or medicament for immunizing against or treating for infection by invertebrate endoparasites or ectoparasites comprising an immunologically effective amount of antibodies produced from hybridomas of the cell line ATCC 9529 which specifically bind to the native form of juvenile hormone and a pharmaceutically acceptable carrier.

### Claim 8 (depends on 7)

A method of immunizing a vertebrate against or treating a vertebrate for infection by endoparasites or ectoparasites comprising administering to the vertebrate an immunologically effective amount of a composition of claim 7.

### Claim 9

A pharmaceutical composition for use in a host vertebrate as a vaccine or medicament for immunizing against or treating for infection by invertebrate endoparasites or ectoparasites comprising an immunologically effective amount of antibodies produced from hybridomas of the cell line ATCC 9530 which specifically bind to the native form of beta-ecdysone and a pharmaceutically acceptable carrier.

### Claim 10 (depends on 9)

A method of immunizing a vertebrate against or treating a vertebrate for infection by endoparasites or ectoparasites comprising administering an immunologically effective amount of the composition of claim 9.

### Claim 11

A method of immunizing a vertebrate against or treating a vertebrate for infection by endoparasites or ectoparasites comprising administering to the vertebrate an immunologically effective amount of a first composition comprising antibodies that specifically bind to the native form of juvenile hormone and an immunologically effective amount of a second composition comprising antibodies that specifically bind to the native form of beta-ecdysone.