Gelonin immunotoxin
AssigneeDana-Farber Cancer Institute, Inc.
InventorsJohn M. Lambert, Walter A. Blattler, Peter D. Senter
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Abstract
Immunotoxins having improved toxic activity have the composition ##STR1## wherein Toxin-NH is a ribosome-inactivating protein containing no accessible sulfhydryl groups, n is an integer from 1 to 5, m is an integer from 1 to 5, and NH-Antibody is a monoclonal antibody specific to eucaryotic cells or antigens associated therewith. The immunotoxins are made by reacting the Toxin-NH with an iminothiolester salt to form a first conjugate, reacting the NH-Antibody with N-succinimidyl-3-(2-pyridyldithio) propionate to form a second conjugate, and reacting the two conjugates to form the immunotoxin.
Claims10
An immunotoxin having the structure ##STR5## wherein Toxin-NH is gelonin, n is an integer from 1 to 5, m is an integer from 1 to 5, NH-Antibody is a monoclonal antibody specific to eucaryotic cells or to antigens associated therewith, and A.sup.- is a non-toxic water-soluble anion.
Dependencies are inferred from the claim text.