Detection of reticulocytes, RNA or DNA
AssigneeBecton, Dickinson and Company
InventorsLinda G. Lee, Chia-Huei Chen
Life of this patent
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Abstract
Reticulocytes, RNA or DNA are stained with a dye for detection in a flow cytometer. The dye has the formula: ##STR1## Wherein: X=O,S, Se, N -alkyl (having 1-6 carbons) or C (CH.sub.3).sub.n ; R.sub.1 =alkyl having from 1-6 carbons; R.sub.2 =alkyl having from 1-6 carbons; R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is absent; R.sub.4 =alkyl having 1-6 carbons, methoxy or is absent; and n=zero or an integer from 1-6.
Claims15
A compound composed of reticulocytes bound to a dye having the formula:
- ##STR7## Wherein:
- X.dbd.O,S, Se or C (CH.sub.3).sub.2 :
- R.sub.1 =alkyl having from 1-6 carbons;
- R.sub.2 =alkyl having from 1-6 carbons;
- R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or hydrogen;
- R.sub.4 =alkyl having 1-6 carbons, methoxy or hydrogen;
- and
- n=zero or an integer from 1-6.
A compound composed of RNA bound to a dye having the formula:
- ##STR8## Wherein:
- X.dbd.O,S, Se or C (CH.sub.3).sub.2 :
- R.sub.1 =alkyl having from 1-6 carbons;
- R.sub.2 =alkyl having from 1-6 carbons;
- R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is hydrogen;
- R.sub.4 =alkyl having 1-6 carbons, methoxy or hydrogen;
- and
- n=zero or an integer from 1-6.
A compound composed of DNA bound to a dye having the formula:
- ##STR9## wherein:
- X.dbd.O,S, Se or C (CH.sub.3).sub.2 ;
- R.sub.1 =alkyl having from 1-6 carbons;
- R.sub.2 =alkyl having from 1-6 carbons;
- R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is hydrogen;
- R.sub.4 =alkyl having 1-6 carbons, methoxy or hydrogen;
- and
- n=zero or an integer from 1-6.
Dependencies are inferred from the claim text.