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US 4,879,234 AGranted
Process for the purification and recovery of formate-dehydrogenase (FDH) from Candida boidinii, and FDH-containing product
AssigneeGesellschaft fur Biotechnologische Forschung mbH (GBF)
InventorsArno Cordes, Maria-Regina Kula, Karl-Heinz Kroner, Wolfgang Stach
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Abstract
The invention relates to a process for the purification and, if desired, recovery of formate-dehydrogenase (FDH) from Candida boidinii, in which FDH is subjected to a phase distribution in an aqueous 2-phase system that contains a triazine dyestuff that is bonded to an inert water-soluble polymer.
Claims14
Process for the purification and recovery of formate-dehydrogenase enzyme from Candida boidinii cells, which comprises:
- (a) disintegrating the enzyme-containing cells to obtain a mixture of cell material and the enzyme;
- and
- (b) affinity extracting the enzyme from the mixture with an aqueous 2-phase extracting agent, one phase of which contains a triazine dyestuff bonded to an inert, water-soluble polymer.
Process for the purification and recovery of formate-dehydrogenase enzyme from Candida boidinii, which comprises;
- providing frozen cells of Candida boidinii;
- suspending the cells in an aqueous medium containing a salt selected from the group consisting of potassium phosphate, potassium hydrogen phosphate and mixture thereof;
- leaving the cells suspended in the solution until an enzyme efflux of a maximum of approximately 90 to 75 percent activity is achieved;
- and
- affinity extracting the enzyme from the solution with an aqueous 2-phase extracting agent, in one phase of which there is a concentrated triazine dyestuff bonded to an inert, water-soluble polymer.
2 more elements
Dependencies are inferred from the claim text.