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US 4,879,219 AGranted
Immunoassay utilizing monoclonal high affinity IgM antibodies
AssigneeThe General Hospital Corporation
InventorsJack R. Wands, Vincent R. Zurawski, Jr., Schoemaker, Hubert J. P.
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Abstract
Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of viral antigens.
Claims9
An immunoassay method utilizing an antibody to assay for a substance comprising hepatitis B-surface antigen (HBsAg) determinants which comprises the steps of:
- contacting a test sample containing said substance comprising HBsAg determinants with said antibody;
- and
- determining the presence of said substance in said sample;
- wherein said antibody is a monoclonal high affinity IgM antibody having a binding affinity constant for said HBsAg determinants of at least 10.sup.9 M.sup.-1.
1 more element
Monoclonal high affinity IgM antibodies immunoreactive with HBsAg determinants wherein said antibodies are coupled to an insoluble solid phase, and wherein the binding affinity constant of said antibodies for said HBsAg determinants is at least 10.sup.9 M.sup.-1.
Dependencies are inferred from the claim text.