Feline leukemia virus protein vaccines
AssigneeChiron Corporation
InventorsPaul Luciw, Deborah L. Parkes, Gary A. Van Nest
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Abstract
Methods and compositions are provided for the efficient production of a polypeptide having immunological reactivity corresponding to that of naturally-occurring envelope glycoproteins of feline leukemia virus. A DNA construct including a replication system recognized by yeast, and a viral envelope protein gene under the transcriptional control of a yeast promoter, and terminator is provided. By transforming a yeast host with the DNA construct, enhanced yields of the product may be obtained. Saccharomyces carlsbergensis strains 2150-2-3 (pCP-envB-R) and 2150-2-3 (pCP-envA-R) were deposited at the American Type Culture Collection on July 18, 1984, and granted accession nos. 20720 and 20721, respectively.
Claims13
A polypeptide capable of inducing an immune response to produce antibodies specific for feline leukemia virus (FeLV) envelope glycoprotein, said polypeptide having been produced in a unicellular microorganism host and having the glycosylation pattern imposed by said unicellular host.
FeLV envelope glycoprotein isolated from yeast transformed with a DNA construct comprising a DNA fragment coding for the amino acid sequence of at least one epitope of a feline leukemia virus (FeLV) envelope glycoprotein under transcriptional control of promoter and terminator signals recognized by yeast, and a replication system recognized by yeast.
Dependencies are inferred from the claim text.