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AssigneeSolvay & Cie
InventorsWilliam H. Andrews, Virginia M. Brothers, James G. Files, Irene Kuhn, Michael T. McCaman, Leland S. Paul, Stacey R. Sias, Thomas C. Gore, Karel Z. Newman, Jr., John L. Tedesco
Estimating the term…
A genomic DNA molecule having the nucleic acid sequence set forth in FIG. 1 and encoding an antigenic protein derived from Eimeria tenella has been isolated. The protein has a molecular weight of about 25,000 daltons and is composed of two polypeptides joined by a disulfide bond. One of the polypeptides is characterized by a molecular weight of about 17,000 daltons and by a blocked N-terminal amino acid and having the amino acid sequence set forth in FIG. 1. The other polypeptide is characterized by a molecular weight of about 8,000 daltons and has the amino acid sequence set forth in FIG. 1. A cDNA molecule encoding the 25,000 dalton polypeptide with a continuous amino acid sequence has been inserted into expression vectors capable of expressing the 25,000 dalton polypeptide directly or as a fused polypeptide. The polypeptides produced are used in vaccines to immunize chickens against infection by Eimeria tenella.
An isolated genomic DNA molecule having the mucleic acid sequence set forth in FIG. 1 and encoding an antigenic protein derived from Eimeria tenella, the protein having a molecular weight of about 25,000 daltons and being composed of two polypeptides joined by a disulfide bond, one of the polypeptides being characterized by a molecular weight of about 17,000 daltons and by a blocked N-terminal amino acid and having the amino acid sequence set forth in FIG. 1 and the other of the polypeptides being characterized by a molecular weight of about 8,000 daltons and having the amino acid sequence set forth in FIG. 1.
A nucleic acid molecule encoding an antigenic polypeptide, the polypeptide having a molecular weight of about 25,000 daltons and having the continuous amino acid sequence set forth in FIG. 4.
An E. coli host cell designated REN3/pBGC23 which comprises the vector pBGC23 and has ATCC accession No. 53317.
An E. coli host cell designated REN3/pCOC12, which comprises the vector pCOC12 and has ATCC accession No. 53314.
An E. coli host cell designated REN3/pCOC20, which comprises the vector pCOC20 and has ATCC accession No. 53313.
An E. coli host cell designated REN3/pDET1, which comprises the vector pDET1 and has ATCC accession No. 53316.
An E. coli host cell designated REN3/pDET2, which comprises the vector pDET2 and has ATCC accession No. 53318.
Dependencies are inferred from the claim text.