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US 4,865,984 AGranted
Dynamic continuous flow enzyme reactor
AssigneeMount Sinai School of Medicine of the City University of New York
InventorsYale Nemerson, Vincent Turitto
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AssigneeMount Sinai School of Medicine of the City University of New York
InventorsYale Nemerson, Vincent Turitto
Estimating the term…
A dynamic continuous flow enzyme reactor and method for carrying out and analyzing phospholipid-dependent enzyme reactions are provided. The enzyme reactor is a tubular member preferably a capillary tube, coated on its inner surface with a planar phospholipid bilayer membrane, optionally and preferably containing an enzyme or enzyme cofactor. The reactor is connectable at one end to a means for delivering fluid flow reagents to the reactor and is connectable at a second end to a means for analyzing an effluent exiting from the reactor. Preferably the enzyme cofactor in the phospholipid membrane is purified tissue factor and the fluid reagents are inactive blood clotting factors which become activated upon interaction with the phospholipid membrane through a tissue factor-mediated reaction.
A dynamic continuous flow enzyme reactor for carrying out and analyzing phospholipid-dependent enzymatic reactions which comprises a tubular housing coated on its inner surface with a planar phospholipid bilayer membrane, the housing being connectable at one opening to a means for delivering fluid flow reagents thereto and connectable at a second opening to a means for collecting and analyzing an effluent fluid therefrom.
A method for continuously carrying out and measuring dynamic enzyme reactions comprising:
Dependencies are inferred from the claim text.