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US 4,861,866 AGranted
Continuous flow peptide synthesizer
AssigneeEldex Laboratories, Inc.
InventorsEmmett L. Durrum, Stephen Amendola, Bruce W. Erickson
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AssigneeEldex Laboratories, Inc.
InventorsEmmett L. Durrum, Stephen Amendola, Bruce W. Erickson
Estimating the term…
A continuous flow peptide synthesizer (10) has reservoirs (12-42) for amino acid derivatives and reservoirs (154-156 and 161-167) for peptide synthesis reagents and solvents. A column (44) has column segments (224, 226, 228) forming stacked interconnected chambers for holding peptide synthesis polymeric resin supports (260). Each of the amino acid derivative reservoirs (12-42) and the reagent and solvent reservoirs (152-154 and 161-167) are connected through a separate controllable valve (46-76, 154-156 or 182-194) to the column (44). A pressurized source (106) of helium or other inert gas is provided for transferring the amino acid derivatives, reagents and solvents to the column (44). The column segments (224) are also used in a vessel (270) for confining the polymeric resin supports (260) in the vessel during cleavage of the peptides from the supports (260).
A continuous flow peptide synthesizer, which comprises a first plurality of reservoirs for amino acid derivatives, a second plurality of reservoirs for peptide synthesis reagents, a column having a third plurality of separate column segments forming stacked, serially interconnected chambers for holding peptide synthesis polymeric resin supports, said separate column segments each having an axially extending bore with at least one mesh screen extending across said axially extending bore, said stacked chambers being formed between said mesh screens when said separate column segments are assembled in stacked relationship, each of said first plurality of amino acid derivative reservoirs and said second plurality of reagent reservoirs being connected through a separate controllable valve to said column, and means for supplying the amino acid derivatives and the reagents simultaneously to each of said separate column segments in said column.
A peptide synthesizer column, which comprises a housing configured to support a plurality of separate column segments in stacked relationship to form a serially interconnected plurality of stacked chambers, said separate column segments each having an axially extending bore with at least one mesh screen extending across said axially extending bore, said stacked chambers being formed between said mesh screens when said separate column segments are assembled in stacked relationship, said housing having at least one member movable along its length to allow said housing to support a variable number of said separate column segments in the stacked, serially connected relationship, said plurality of separate column segments including a separate inlet column segment, a separate outlet column segment and at least one additional separate column segment between said separate inlet column segment and said separate outlet column segment.
A column segment, which comprises a cylinder having an axially extending bore with at least one mesh screen extending across said axially extending bore, said cylinder being configured to mate with a like cylinder in separate and stacking relationship to form serially interconnected, separate stacked chambers, said separate stacked chambers being formed between said mesh screens when said column segments are assembled in separate and stacked relationship.
A continuous flow peptide synthesis process, which comprises providing a plurality of peptide synthesis polymeric resin support bodies separably stacked in serially interconnected relationship in a column, flowing peptide synthesis reagents for a plurality of synthesis steps which are identical for a plurality of resin support bodies simultaneously through the stacked bodies in the column, separating the stacked bodies for at least one synthesis step which is different for the resin support bodies, and flowing at least one different peptide synthesis reagent for each separated resin support body through the separated resin support bodies.
Dependencies are inferred from the claim text.