Novel receptors for efficient determination of ligands and their antagonists or agonists
AssigneeGenentech, Inc.
InventorsThomas J. Dull, Heimo Riedel, Axel Ullrich
Life of this patent
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Abstract
Hybrid receptors are provided that comprise (a) the ligand binding domain of a predetermined receptor and (b) a heterologous reporter polypeptide. The hybrid receptors are useful for convenient and large scale assay of biologically active ligands or their antagonists or agonists.
Claims15
A hybrid receptor for a ligand comprising (a) a ligand binding domain of a predetermined receptor fused at its C-terminus to the N-terminus of (b) a reporter polypeptide which is (1) heterologous to the receptor from which is obtained the ligand binding domain and which (2) undergoes a conformational change upon the binding of said ligand to the ligand binding domain.
A method for assaying a biologically active ligand or an antagonist or agonist for said ligand, comprising
- (a) providing a hybrid receptor which comprises (1) a binding domain for the ligand, antagonist or agonist and (2) a heterologous reporter polypeptide;
- (b) incubating the receptor with a test sample suspected to contain the ligand, antagonist or agonist;
- (c) detecting a conformational change in the reporter polypeptide;
- and
- (d) correlating said change with the presence of the ligand, antagonist or agonist in the test sample.
Dependencies are inferred from the claim text.