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US 4,859,597 AGranted
Production of phage and phage-associated lysin
AssigneeIgene Biotechnology, Inc.
InventorsHuei-Hsiung Yang, Stephen F. Hiu, John L. Harris
Life of this patent
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Abstract
A method for producing a lysin-free phage inoculum, which comprises: (a) inoculating a growing Streptococcal culture with phage, (b) incubating the culture for a plurality of lytic cycles of phage until the cells are completely lysed to obtain a lysate, and (c) removing cell debris and free lysin from the lysate to form a lysin-free phage suitable for use as an inoculum. A method for producing lysin is also disclosed.
Claims16
A method for producing a lysin-free phage inoculum, which comprises:
- (a) inoculating a growing Streptococcal culture with phage,
- (b) incubating the culture for a plurality of lytic cycles of phage until the cells are completely lysed to obtain a lysate, and
- (c) removing cell debris and free lysin from the lysate to form a lysin-free phage suitable for use as an inoculum.
Dependencies are inferred from the claim text.