Immunoassay device

A device for conducting an assay method, said device comprising:

• (a) a housing,
• (b) means enclosed in said housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from said zone wherein said means comprises a first piece of bibulous material that is immunosorbing or is capable of being rendered immunosorbing and a second piece of bibulous material which is in liquid receiving relationship with said first piece of bibulous material,
• (c) one or more liquid reagents confined for subsequent release in at least one breakable container enclosed in said housing for conducting an assay method for the determination of an analyte in a sample, and
• (d) means in said housing for introducing the sample into the device.

A device for conducting an assay method, which device comprises:

• a housing,
• a first bibulous material enclosed in said housing, said bibulous material having a member of a specific binding pair (sbp) non-diffusively bound thereto,
• a second bibulous material enclosed in said housing in liquid receiving relationship to said first bibulous material,
• a liquid reagent for conducting an assay contained in a breakable container,
• an opening in said housing for introducing a sample suspected of containing an analyte into said device, and
• means integral with said housing allowing for breaking said container.

A device for conducting an assay, said device comprising:

• a housing,
• a pad of bibulous material enclosed in said housing having a member of a specific binding pair (sbp) non-diffusively bound thereto,
• a pad of absorbent bibulous material enclosed in said housing,
• a strip of bibulous material enclosed in said housing providing a liquid receiving relationship between said pads,
• a breakable container enclosed in said housing, said container having contained therein a liquid reagent comprising an enzyme substrate, and
• an inlet port for introducing a sample suspected of containing an analyte into said device,
• said housing having a flexible portion adjacent said breakable container.

A device for conducting an assay method, said device comprising:

• (a) a housing,
• (b) means enclosed in said housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action to and away from said zone,
• (c) one or more liquid reagents confined for subsequent release in at least one breakable container enclosed in said housing for conducting an assay method for determination of an analyte in a sample, and
• (d) means in said housing for introducing the sample into the device.

A device for conducting an assay method, which device comprises:

• a housing,
• a first bibulous material enclosed in said housing, said bibulous material having a zone wherein a member of a specific binding pair (sbp) is non-diffusively bound thereto,
• a second bibulous material enclosed in said housing in liquid receiving relationship to said first bibulous material,
• a liquid reagent for conducting an assasy confined for subsequent release in a breakable container enclosed in said housing,
• an opening in said housing for introducing a sample suspected of containing an analyte into said device, and
• means integral with said housing allowing for breaking said container whereupon said liquid reagent is transported by capillary action to and away from said zone of said first bibulous material and into said second bibulous material.