Glutamate receptor inhibitor
AssigneesTokyo Metropolitan Institute for Neurosciences, Takeda Chemical Industries, Ltd.
InventorsMasanori Yoshioka, Hidemitsu Hou, Nobufumi Kawai
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Abstract
A novel glutamate receptor inhibitor obtained from Joro spider venom glands, and its manufacturing method.
Claims6
An insecticidally effective composition comprising a glutamate receptor inhibitor, said glutamate receptor inhibitor having insecticidal properties when present in a concentration of at least 10.sup.-9 M, said glutamate receptor inhibitor possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said glutamate receptor inhibitor being an organic compound obtained from Joro spider venom glands and which has the following properties:
- (a) molecular weight is within the range of 500.+-.200;
- (b) it is substantially insoluble in ethyl acetate and soluble in water;
- (c) the activity remains unchanged when heated to 80.degree. C. for 20 minutes in water;
- (d) the activity remains unchanged when heated to 80.degree. C. for 20 minutes in 0.4 M perchloric acid;
- (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25);
- (f) it develops color with ninhydrin and fluoresces with fluorescamine;
- (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
A glutamate receptor inhibiting compound obtained from Joro spider venom glands, said compound possessing only one single spot at the position of Rf value of substantially 0.20 on a Silica gel 60 thin-layer plate developed with phenol-water mixed solvent 3:1 (V/V), said compound having the following properties:
- (a) molecular weight is within the range of 500.+-.200;
- (b) it is substantially insoluble in ethyl acetate and soluble in water;
- (c) the activity remains unchanged when heated to 80.degree. C. for 20 minutes in water;
- (d) the activity remains unchanged when heated to 80.degree. C. for 20 minutes in 0.4 M perchloric acid;
- (e) it shows in electrophoresis neutral electric charge with use of 0.01 M phosphate buffer (pH 7) and positive electric charge using 0.2 M formate buffer (pH 2.25);
- (f) it develops color with ninhydrin and fluoresces with fluorescamine;
- (g) it shows maximum absorption substantially at 270 nm, and minimum absorption substantially at 245 nm in water (pH 6.1).
An insecticidal composition of claim 2 comprising an amount of the glutamate receptor inhibitor of claim 8 in a concentration of at least 10 ppm and an agriculturally or horticulturally acceptable carrier therefor.
Dependencies are inferred from the claim text.