Water-compatible reducible compounds and their use in analytical compositions and methods
AssigneeEastman Kodak Company
InventorsAlbert J. Mura, Robert T. Belly, Vanessa R. Lum
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Abstract
Certain water-compatible reducible compounds are useful in analytical compositions and elements for assay of various analytes, e.g. microorganisms. These compounds comprise a moiety which provides a detectable species (e.g. a dye) when released from the compound at physiological pH. Further, these compounds are aromatic derivatives or quinones having water-compatibilizing substituents which allow them to be used in compositions without the use of surfactants.
Claims16
A water-compatible reducible compound of the structure CAR--(R.sup.1).sub.n wherein CAR-- is a substituted or unsubstituted aromatic or quinone nucleus, R is ##STR32## wherein R--.sup.5 is substituted or unsubstituted alkylene of 1 to 2 carbon atoms, R--.sup.6 is substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl or substituted or unsubstituted aryl, Q is carbonyl or thiocarbonyl, m is 0 or 1, FRAG is a chromogen or fluorogen and n is 1 or 2,
- provided said compound is constructed such that it is capable of being reduced at a pH of 9 or less to release said FRAG, and said compound comprises at least one water-compatibilizing moiety which has a hydrophobic parameter less than about -2.0, and
- further provided that when R.sup.1 is replaced with H, CAR--(H).sub.n is constructed such that it has an E.sub.1/2 of at least about +100 mV when measured in water.
An aqueous composition buffered at a pH of 9 or less and comprising a water-compatible reducible compound of the structure CAR--(R.sup.1).sub.n wherein CAR-- is a substituted or unsubstituted aromatic or quinone nucleus, R is ##STR35## where R--.sup.5 is substituted or unsubstituted alkylene of 1 to 2 carbon atoms, R--.sup.6 is substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl or substituted or unsubstituted aryl, Q is carbonyl or thiocarbonyl, m is 0 or 1, FRAG is a chromogen or fluorogen, and n is 1 or 2,
- provided said reducible compound is constructed such that it is capable of being reduced at a pH of 9 or less to release said FRAG, and said compound comprises at least one water-compatibilizing moiety which has a hydrophobic parameter less than about -2.0, and
- further provided that when R.sup.1 is replaced with H, CAR--(H).sub.n is constructed such that it has an E.sub.1/2 of at least about +100 mV when measured in water.
A dry analytical element for the determination of an analyte comprising an absorbent carrier material, and containing a water-compatible reducible compound of the structure CAR--(R.sup.1).sub.n wherein CAR is a substituted or unsubstituted aromatic or quinone nucleus, R is ##STR38## wherein R--.sup.5 is substituted or unsubstituted alkylene of 1 to 2 carbon atoms, R--.sup.6 is substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl or substituted or unsubstituted aryl, Q is carbonyl or thiocarbonyl, m is 0 or 1, FRAG is a chromogen or fluorogen, and n is 1 or 2,
- provided said compound is constructed such that it is capable of being reduced at a pH of 9 or less to release said shiftable detectable species, and said compound comprises at least one water-compatibilizing moiety which has a hydrophobic parameter less than about -2.0, and
- further provided that when R.sup.1 is replaced with H, CAR--(H).sub.n is constructed such that it has an E.sub.1/2 of at least about 30 100 mV when measured in water.
Dependencies are inferred from the claim text.