Method and composition for the detection and diagnosis of Legionella pneumophila
AssigneeGenetic Systems Corporation
InventorsLynn C. Goldstein, Larry H. Gosting
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Abstract
Method and compositions including monoclonal antibodies to a serogroup-common antigen are provided for the detection and diagnosis of Legionella pneumophila. The monoclonal antibodies recognize a proteinaceous antigen of molecular weight 28,000-29,000 Daltons which is detected in at least serogroups 1 through 8 of Legionella pneumophila and is not detected in other common respiratory pathogens.
Claims29
A method for the detection and diagnosis of the bacterium Legionella pneumophila in biological specimens, said method comprising reacting a sample suspected of containing said bacterium with a labeled monoclonal antibody to a Legionella pneumophila proteinaceous serogroup-common antigen, wherein said antibody does not react with antigens of other Legionella species, and detecting the presence of a signal provided by said label.
Monoclonal antibodies which react with an antigenic determinant on an antigen common to at least serogroups 1 through 8 of Legionella pneumophila and which determinant is not present in Pseudomonas fluorescens, Legionella bozemanii, Legionella micdadei, Legionella dumoffii, Legionella longbeachae 1 and 2, Legionella jordanis, Legionella Gourmanii, Legionella oakridgensis, or Legionella sainthelinsi.
Monoclonal antibodies which are capable of specifically recognizing and bindigg to an antigen derived from Legionella pneumophila, said antigen characterirzed in that it:
- a. is present in at least serogroups 1 through 8 of Legionella pneumophila;
- b. is not present in other legionella species;
- c. has a molecular weight between 28,000 and 29,000;
- and
- d is proteinaceous.
The hybrid cell line identified as LP3-IIG2 (ATCC-HB-8472).
An antigen isolated from Legionella pneumophila, which antigen:
- a. is present in at least serotypes 1 through 8 of Legionella pneumophila;
- b. is not present in Pseudomonas fluorescens, Legionella bozemanii or Legionella micdadei;
- c. has a molecular weight between 28,000 and 29,000;
- d. is susceptible to degradation by proteinase K.
Monochlonal antibodies reactive with an antigenic determinant which is capable of being recognized and specifically bound by monoclonal antibodies identified as LP3-IIG2 (ATCC HB 8472).
Dependencies are inferred from the claim text.