Enzyme immobilization with a hydrolyzed polysaccharide graft copolymer
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsGeorge Fanta, Patricia J. Slininger
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Abstract
Immobilized enzyme-polysaccharide graft copolymer products are prepared by hydrolyzing a polysaccharide graft copolymer and then contacting the hydrolyzed copolymer with an enzyme to immobilize the enzyme on the copolymer. The copolymer-enzyme products retain large quantities of active enzyme, and activity of the enzyme is retained over long periods of use. In the case of glucoamylase immobilized in accordance with the method of the invention, improved yield of glucose from starch is obtained.
Claims14
A method for preparing a polysaccharide graft copolymer-enzyme product, which consists essentially of:
- (a) hydrolyzing a polysaccharide graft copolymer, prepared by reaction of a vinyl or acrylic monomer with a polysaccharide, to remove a portion of said polysaccharide in said polysaccharide graft copolymer and obtain a final polysaccharide content in said hydrolyzed polysaccharide graft copolymer of 2 to 70%;
- and
- (b) contacting said hydrolyzed polysaccharide graft copolymer with an enzyme for a time sufficient to bind the enzyme to the copolymer.
Dependencies are inferred from the claim text.