Method for the determination of sarcosine creatine or creatinine
AssigneeBoehringer Mannheim GmbH
InventorsUlrich Mayr, Hans Mollering, Joachim Siedel, Hans Seidel
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Abstract
The present invention provides a method of use for a hydrogen peroxide-forming oxidase, wherein the enzyme is obtained from Streptomycetaceae and at 25.degree. C., in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity. This enzyme is useful in the determination of sarcosine, creatine and creatinine.
Claims8
A method for the determination of sarcosine which comprises:
- (1) obtaining hydrogen peroxide-forming sarcosine oxidase, from Streptomycetaceae wherein said enzyme at 25.degree. C. in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity;
- (2) reacting a sample containing sarcosine with the sarcosine oxidase obtained in step 1), and
- (3) measuring the H.sub.2 O.sub.2 produced.
Method for the determination of creatine which comprises:
- (1) obtaining hydrogen peroxide-forming sarcosine oxidase, from streptomycetaceae wherein said enzyme 25.degree. C. in 0.15 mol/litre potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40 of the initial activity;
- (2) reacting a sample containing creatine with creatineamidinohydrolase whereby sarcosine and urea are formed and further reacting the sarcosine with the sarcosine oxidase obtained in step 1, and
- (3) measuring the H.sub.2 O.sub.2 produced.
Method for the determination of creatinine which comprises:
- (1) obtaining hydrogen peroxide-forming sarcosine oxidase, from Streptomycetaceae wherein said enzyme at 25.degree. C. in 0.155 mol/litre potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity;
- (2) reacting creatinine with creatininamidohydrolase to form creatine, reacting the so-formed creatine with creatinamidinohydrolase to form sarcosine and urea, andn further reacting the sarcosine with the sarcosine oxidase obtained in step 1, and
- (3) measuring the H.sub.2 O.sub.2 produced.
Dependencies are inferred from the claim text.