Chromatography system using horizontal flow columns
AssigneeSepragen Corporation
InventorVinit Saxena
Life of this patent
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Abstract
A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures. The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ion-exchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques.
Claims22
A low pressure chromatography system using at least one horizontal column, said system comprising:
- at least one chromatographic column having a bed of separation medium located intermediate an inlet channel and an outlet channel said inlet and outlet channels being coaxially positioned with respect to each other, said column being constructed such that sample fluid passes through said bed in a horizontal direction from said inlet channel to said outlet channel,
- means for directing sample fluid to said column including pump means having an outlet connected via a first fluid line to an inlet of said column, said inlet being in fluid communication with said inlet channel, and valve means mounted in said first fluid line for controlling passage of fluid therethrough,
- detection means connected to said column for detecting sample fluid fractions discharged from said outlet channel of said column, and
- means for controlling at least sample fluid directed into said inlet of said column and sample fluid fractions discharged from said outlet channel of said column.
Dependencies are inferred from the claim text.