Cell culture flask utilizing a membrane barrier
OwnerJoseph G. Cremoneseinventor
Life of this patent
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Abstract
A cell culture flask includes a hollow housing and a gas permeable membrane disposed within the housing which divides the housing into a first chamber and a second chamber. A retaining ring secures the gas permeable membrane within the housing and forms a liquid-tight seal between the first chamber and the second chamber. A gas inlet in the housing communicates with the first chamber and supplies gas thereto. A media inlet in the housing communicates with the second chamber and provides cell growth media thereto.
Claims28
A cell culture flask comprising a hollow housing, a gas permeable and liquid impervious membrane disposed within said housing and dividing said housing into a first chamber and a second chamber, retaining means for removably securing said gas permeable membrane within said housing and forming a liquid-tight seal between said first chamber and said second chamber, a gas inlet into said housing and communicating with said first chamber for supplying gas thereto, and a media inlet into said housing and communicating with said second chamber for supplying cell growth media thereto.
A cell culture flask comprising:
- an open-topped, hollow housing having a base and a peripheral wall;
- a gas permeable and liquid impervious membrane disposed within said housing above said base and dividing said housing into a first chamber and a second chamber;
- a removable retaining ring conforming to an inner geometric diameter of said peripheral wall, said membrane being frictionally held in place between an outer surface of said retaining ring and an inner surface of said housing whereby a liquid-tight seal is formed between said first chamber and said second chamber;
- a gas inlet and a gas outlet into said housing and communicating with said first chamber;
- and
- a lid removably positioned on said housing and spaced above said membrane, with said lid having a media inlet and a media outlet therethrough and communicating with said second chamber.
A cell culture flask comprising:
- an open-topped hollow housing that includes a base and a peripheral wall;
- a gas permeable and liquid impervious membrane disposed over the open top and said peripheral wall of said housing;
- a retaining ring for removably securing the gas permeable membrane about an outer surface of said peripheral wall, said retaining ring conforming to an outer geometric diameter of said peripheral wall, said membrane being frictionally held in place by compressing an outer perimeter of said membrane between an inner surface of said retaining ring and an outer surface of said housing, and said retaining ring extending above said peripheral wall;
- a first chamber formed below said membrane;
- a second chamber formed above said membrane and within the inner surface of the retaining ring;
- and
- a gas inlet into said housing and communicating with said first chamber for supplying gas thereto.
An apparatus for cell culture growth comprising:
- a cell culture flask including a hollow housing, a gas permeable and liquid impervious membrane disposed within said housing and dividing said housing into a first chamber and a second chamber, a retaining means for removably securing said gas permeable membrane within said housing and forming a liquid-tight seal between said first chamber and said second chamber;
- a gas inlet into said housing and communicating with said first chamber for supplying gas thereto;
- and a media inlet into said housing and communicating with said second chamber for supplying cell growth media thereto;
- an incubator for receiving one or more of said culture flasks, said incubator having means to permit gas exiting from said flask to exit said incubator, said incubator having means for supplying media to each of said flasks and said incubator having means for supplying gas to each of said flasks;
- at least one gas source under pressure;
- means for blending the gas and selectively controlling the gas flow to said flasks;
- and
- a pump for depositing liquid media into said flasks.
Dependencies are inferred from the claim text.