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US 4,839,282 AGranted
Preparation of 3-oxo-.DELTA..sup.1,4 -steroids
AssigneeSchering Corporation
InventorsAlfred Weber, Mario Kennecke
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Abstract
A process for preparing a 3-oxo-.DELTA..sup.1,4 -steroid comprises fermenting the corresponding 3-oxo-.DELTA..sup.4 -steroid, saturated in the 1,2-position, witih a living culture of Arthrobacter simplex in the presence of 0.04 g to 0.12 g of cobalt(II) ions per liter of culture broth.
Claims6
A process for the preparation of a 3-oxo-.DELTA..sup.1,4 -steroid of the formula ##STR6## wherein X is hydrogen, chlorine, fluorine or methyl, ##STR7## wherein Y is hydrogen, fluorine, or chlorine, and ##STR8## wherein Z is hydrogen, hydroxy, fluorine, or chlorine,
- R.sub.1 is hydrogen or an alicyclic hydrocarbon residue of 1-4 carbon atoms,
- R.sub.2 is hydrogen, hydroxy, alkyl of 1-6 carbon atoms optionally interrupted by an oxygen atom or a sulfur atom, alkanoyloxy of 1-6 carbon atoms, or benzoyloxy,
- R.sub.3 is hydrogen, hydroxy or methyl, and
- R.sub.4 and R.sub.5 each independently is alkyl or 1-4 carbon atoms, comprising
- fermenting a corresponding 3-oxo-.DELTA..sup.4 -steroid of the formula ##STR9## wherein X, ##STR10## are as defined above, or a 21-ester thereof with an alkanecarboxylic acid of 1-6 carbon atoms, with a living culture of Arthrobacter simplex in the presence of 0.04 g to 0.12 g of cobalt(II) ions per liter of culture broth.
2 more elements
Dependencies are inferred from the claim text.