Fibrin-collagen tissue equivalents and methods for preparation thereof
AssigneeOrganogenesis, Inc.
InventorCrispin B. Weinberg
Life of this patent
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Abstract
The present invention provides fibrin-collagen tissue equivalents and methods of making and using such tissue equivalents. The present invention also provides methods of forming multi-layer tissue equivalents having improved adherence of the layers. The present invention further provides a method for joining tears in tissue equivalents.
Claims46
A tissue equivalent comprising (i) a hydrated collagen lattice contracted by a contractile agent and (ii) fibrin.
A tissue equivalent having at least two layers, wherein one or more of the layers comprises (i) a hydrated collagen lattice contracted by a contractile agent and (ii) fibrin.
A method of producing a tissue-equivalent, the method comprising:
- (a) forming a mixture comprising collagen, fibrinogen, an agent which causes the formation of fibrin from fibrinogen and at least one contractile agent under conditions to form a gel having the collagen, the fibrinogen, the agent which causes the formation of fibrin from fibrinogen and the contractile agent dispersed within said gel mixture;
- and
- (b) maintaining the gel prepared in step (a) under conditions which permit contraction of the gel to form a tissue equivalent.
A method of producing a tissue equivalent, the method comprising:
- (a) forming a mixture comprising collagen and at least one contractile agent under conditions to form a gel having the collagen and contractile agent dispersed therein;
- (b) maintaining the gel obtained in step (a) under conditions which permit contraction of the gel to form a tissue equivalent;
- and
- (c) applying fibrinogen and an agent which causes the formation of fibrin from fibrinogen to at least one surface of the tissue equivalent of step (b).
A method of producing a tissue equivalent having at least two layers, the method comprising:
- (a) forming a mixture comprising collagen and at least one contractile agent under conditions to form a gel having the collagen and contractile agent dispersed within the gel and maintaining the gel under conditions which permit contraction of the gel to form a first tissue equivalent;
- (b) applying a mixture comprising fibrinogen and thrombin to the first tissue equivalent;
- and
- (c) contacting a second tissue equivalent produced in accordance with step (a) above with the first tissue equivalent;
- and
- (d) repeating steps (b) and (c) if additional layers of tissue equivalents are desired.
A method of producing a tissue equivalent having at least two layers, the method comprising:
- (a) forming a mixture comprising collagen, at least one contractile agent, fibrinogen and an agent which causes the formation of fibrin from fibrinogen under conditions to form a gel having the collagen, contractile agent, fibrinogen and the agent which causes the formation of fibrin from fibrinogen dispersed within the gel and maintaining the gel under conditions which permit contraction of the gel to form a first tissue equivalent;
- (b) applying a mixture comprising fibrinogen and an agent which causes the formation of fibrin from fibrinogen to the first tissue equivalent;
- and
- (c) contacting a second tissue equivalent produced in accordance with step (a) above with the first tissue equivalent;
- and
- (d) repeating steps (b) and (c) if additional layers of tissue equivalents are desired.
A method of preparing a tissue-equivalent, comprising:
- (a) forming a acidic solution of collagen;
- (b) combining fibrinogen, an agent which causes the formation of fibrin from fibrinogen, at least one contractile agent and nutrient medium with the acidic solution of collagen;
- (c) raising the pH and the temperature of the solution of collagen to a level sufficient to cause the collagen to gel and form a hydrated collagen lattice containing the fibrinogen, the agent which causes the formation of fibrin from fibrinogen, and the contractile agent;
- and
- (d) maintaining the lattice, fibrinogen, the agent which causes the formation of fibrin from fibrinogen, and the contractile agent under conditions sufficient for the agent to cleave fibrinogen to form fibrin and the contractile agent to contract the collagen lattice thereby forming a tissue equivalent.
A method of repairing a defect in a tissue equivalent comprising a hydrated collagen lattice contracted by a contractile agent or (i) a hydrated collagen lattice contracted by a contractile agent and (ii) fibrin, the method comprising:
- (a) applying a mixture comprising fibrinogen and an agent which causes the formation of fibrin from fibrinogen to the defect in the tissue equivalent;
- and
- (b) maintaining the tissue equivalent of step (a) under conditions to permit formation of fibrin.
Dependencies are inferred from the claim text.