Novel saframycin A derivatives and process for producing the same
AssigneeTadashi Arai
InventorsTadashi Arai, Yuzuru Mikami
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Abstract
Disclosed are compounds of the formula ##STR1## wherein R represents hydrogen or C.sub.1 -C.sub.4 -alkyl. These compounds are obtained by fermentation of the known saframycin A producing strain Streptomyces lavendulae No. 314 and by reaction with the amino acids L-tyrosine, L-methionine, glycine and HOOC-(CH)NH.sub.2 -CH.sub.2 -R. When R is hydrogen, saframycin Y.sub.3 and when R is methyl, saframycin Y.sub.d-1 are produced.
Claims15
A process for the preparation of a compound of the formula ##STR4## wherein R represents hydrogen or C.sub.1 -C.sub.4 -alkyl comprising (a) suspending a biomass of a strain of streptomyces capable of producing saframycin A in an aqueous medium;
- (b) adding to said suspension glycine, L-tyrosine, L-methionine, and an amino acid of the formula
- HO.sub.2 CCH(NH.sub.2)--CH.sub.2 --R (II)
- wherein R is as defined above, either as individual amino acids or peptides consisting of amino acids selected from glycine, L-tyrosine, L-methionine, and the amino acid of formula II, or mixtures of said amino acids and said peptides to yield an enriched suspension;
- (c) incubating said enriched suspension;
- (d) filtering the result of step c;
- and
- (e) adding a cyanide salt to the filtrate obtained from step d to result in said compound of formula I.
A process for the preparation of a compound of the formula ##STR5## wherein R represents hydrogen comprising (a) suspending a biomass of a strain of streptomyces capable of producing saframycin A in an aqueous medium;
- (b) adding to said suspension glycine, L-tyrosine, and L-methionine, either as individual amino acids or as peptides consisting of glycine, L-tyrosine, and L-methionine, or mixtures of said amino acids and said peptides to yield an enriched suspension;
- (c) incubating said enriched suspension;
- (d) filtering the result of step c;
- (e) adding a cyanide salt to the filtrate obtained from step d to result in said compound of formula I;
- and
- (f) then isolating the compound of formula I.
A process for the preparation of a compound of the formula ##STR6## wherein R represents hydrogen or C.sub.1 -C.sub.4 -alkyl comprising (a) suspending a biomass of a strain of streptomyces capable of producing saframycin A in an aqueous medium;
- (b) adding to said suspension glycine, L-tyrosine, L-methionine, and an amino acid of the formula
- HO.sub.2 CCH(NH.sub.2)--CH.sub.2 --R (II)
- wherein R is as defined above, either as individual amino acids or peptides consisting of amino acids selected from glycine, L-tyrosine, L-methionine, and the amino acid of formula II, or mixtures of said amino acids and said peptides to yield an enriched suspension;
- and
- (c) incubating said enriched suspension to result in a compound of formula I.
A process for the preparation of a compound of the formula ##STR7## wherein R represents hydrogen comprising (a) suspending a biomass of a strain of streptomyces capable of producing saframycin A in an aqueous medium;
- (b) adding to said suspension glycine, L-tyrosine, and L-methionine, either as individual amino acids or as peptides consisting of glycine, L-tyrosine, and L-methionine, or mixtures of said amino acids and said peptides to yield an enriched suspension;
- and
- (c) incubating said enriched suspension and isolating said compound of formula I wherein R is hydrogen.
Dependencies are inferred from the claim text.