Microbial degradation of hydrocarbons
AssigneeThe B. F. Goodrich Company
InventorAmikam Horowitz
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Abstract
The subject invention concerns a novel plasmid and its use in a microbial host to degrade a variety of organic compounds. Some of these compounds, such as ethylene dichloride, are undesirable waste products found in various dump sites. The invention also concerns a novel microbe hosting the novel plasmid. The novel plasmid has been shown to encode the gene(s) responsible for the degradation of the organic compounds. Thus, microbes hosting this plasmid, denoted pEDC, can be used to degrade ethylene dichloride, and other compounds.
Claims3
A biologically pure culture of Alcaligenes denitrificans ss. denitrificans, having the identifying characteristics of BFG668.7, which has the property of degrading chloro-aliphatic organic compounds.
Plasmid pEDC, having the following restriction enzyme reactions:
- ______________________________________ PstI cut BamHI cut EcoRI cut XhoI cut (a) bp (a) bp (a) bp (a) bp ______________________________________ 616 9,100 558 12,500 548 14,600 530 19,800 661 7,400 574 11,200 572 11,500 565 11,900 707 6,050 678 6,800 690 6,400 685 6,600 738 5,400 738 5,400 713 5,900 970 2,520 772 4,850 766 4,900 750 5,200 840 3,800 (b) 912 2,950 (b) 809 4,220 960 2,600 1004 2,300 851 3,650 1034 2,150 1178 1,480 901 3,100 ______________________________________
- and which encodes the gene(s) responsible for the degradation of chloro-aliphatic compounds.
A microbe hosting plasmid pEDC.
Dependencies are inferred from the claim text.