Method of measuring concentration of substances
AssigneeOlympus Optical Co., Ltd.
InventorAkihiro Namba
Life of this patent
Estimating the term…
Abstract
A concentration of antigen substances contained in a sample is measured by detecting light scattered by latex particles coated with antibody substances which are specifically reactive with the antigen substances to be measured. The scattered light is received by a photodetector and an output signal of the photodetector is supplied to a fast Fourier transform unit to derive a power spectrum density of the scattered light. Then a white level of the power spectrum density is derived, and the concentration of the antigen substances in the sample is detected from the white level with the aid of a calibration curve representing a relationship between the concentration and the white level.
Claims10
A method of measuring a concentration of substances contained in a sample on the basis of complexes which are formed by a specific reaction between the sample substances and reagent substances which are specifically reactive with said sample substances, the method comprising the steps of:
- projecting a radiation beam upon said complexes;
- detecting radiation scattered by said complexes to produce an output signal;
- processing said output signal to derive a power spectrum density of radiation scattered by said complexes;
- deriving a white level or white level ratio of the power spectrum density, said white level being a value of said power spectrum density which is substantially flat over a low frequency range, and said white level ratio being a ratio of two white levels which are measured at two different times;
- and
- measuring a concentration of the sample substances on the basis of the white level or white level ratio.
Dependencies are inferred from the claim text.