Biocompatible polyorganosiloxane composition for cell culture apparatus
OwnerAlbert J. Banesinventor
Life of this patent
Estimating the term…
Abstract
A polyorganosiloxane composition having a biocompatible surface thereon is disclosed. The biocompatible surface results from the derivatization, or amination, of the surface intended for cell contact. More specifically, the present invention is a polyorganosiloxane composition in which the surface is either treated with a primary amine and optional peptide or the surface is co-cured with a primary amine-containing silane or siloxane. The aminated polyorganosiloxane has utility as a cell culture substrate or in a variety of artificial organ applicatons such as breast implants, synthetic blood vessels, joints, tendons and heart valves. A vacuum apparatus for use with specialized cell cultrue plates incorporating the biocompatible polyorganosiloxane composition is also disclosed.
Claims18
A method for treating the surface of a polyorganosiloxane composition comprising the steps of:
- (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid and hydrobromic acid and then decanting said acid;
- (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine;
- and
- (c) washing said polyorganosiloxane surface with water.
A method for treating the surface of a polyorganosiloxane composition, comprising the co-curing of a polyorganosiloxane composition with an adjacent layer of a compound selected from the group consisting of primary amine-containing silanes, carboxyl-containing silanes, primary amine-containing siloxanes and carboxyl-containing siloxanes.
A cell culture substrate comprising:
- solid means for supporting a cell culture having at least one surface further comprising a polyorganosiloxane composition wherein a surface of said polyorganosiloxane composition is treated by:
- (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid, and hydrobromic acid, and then decanting said acid;
- (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine;
- and
- (c) washing said polyorganosiloxane surface with water.
Dependencies are inferred from the claim text.