Method and kit having layered device for detecting biological component by interference color
AssigneeTeijin Limited
InventorsTakeyuki Kawaguchi, Takashi Shiro
Life of this patent
Estimating the term…
Abstract
A method for detecting a component of a biological system, which comprises contacting a biological component detecting device composed of a light reflecting substrate (I) substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), with a solution presumed to contain the biological component to be detected, then forming a light-transmitting reflecting layer (IV) on its surface, and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
Claims28
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject thc surface layer of the device to contrast enhancement treatment;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A method for detecting a component of a biological system, which comprises providing a biological component detecting device composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III);
- contacting said device with a solution containing the biological component to be detected;
- further reacting another substance capable of reacting with another reaction site in the biological component to be detected after or while reacting the detecting substance with the biological component to subject the surface layer of the device to contrast enhancement treatment;
- then forming a light-transmitting reflecting layer (IV) on its surface, wherein said layer (IV) is a layer of a metal;
- and thereafter detecting the color of light interference or the brightness of the reflected light on the surface of the device.
A kit for detection of a component of a biological system, comprising (I) a pack of a device for detecting the biological component composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), wherein the light interference layer (a) does not have substantially reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
A kit for detection of a component of a biological system, comprising (1) a pack of a device for detecting the biological component composed of a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III), and (2) a pack of a metal colloid, and optionally (3) a pack of a contrast enhancement agent.
A device for detecting a component of a biological system, comprising a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (II), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III).
A device for detecting a component of a biological system, comprising a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a hydrophobizing agent layer (P) formed on the layer (II), and a layer (III) of a substance for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800 nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III).
A device for detecting a component of a biological system, comprising a light reflecting substrate (I), made of a metal or metal alloy itself or having a surface of a metal or metal alloy, and being substantially free from diffused reflection, a light interference layer (II) formed on the substrate (I), a reactive interlayer (P) formed on the layer (II), and a layer (III) of a substrate for detecting said biological component provided at least in a region on the layer (P), wherein the light interference layer (a) does not have substantial reflecting characteristics to visible light, namely a wavelength of 300 to 800nm, (b) has a thickness and refractive index controlled such that an increase in the thickness of the layer (III) appears as a change in interference color, and (c) has a surface with sufficient affinity for the layer (III).
Dependencies are inferred from the claim text.