Immobilized Mucor miehe lipase for transesterification
AssigneeNovo Industri A/S
InventorPeter Eigtved
Life of this patent
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Abstract
Immobilized lipase is produced by mixing an aqueous lipase solution with a particulate, macroporous, weak anion exchange resin, and recovering and drying the resin having lipase immobilized thereon. The resin has a particle size such that more than 90% resin particles have a size between 100-1000 .mu.m. The immobilized lipase is used in a packed bed for continuous transesterification or solvent free fats. Preferably, the lipase is Mucor miehei lipase.
Claims9
A method for production of an immobilized lipase preparation adapted for interesterification of fats, which comprises contacting an aqueous solution of Mucor miehei lipase with a particulate, macroporous, weak anion exchange resin having a particle size such that more than 90% of the resin particles have a size between 100 and 1000 .mu.m, at a pH in range of 5-7 to bind said lipase to said resin to form said immobilized lipase whereby at least 75% of the lipase activity is removed from the aqueous lipase solution to form a spent aqueous solution, then separating the immobilized lipase from the spent aqueous solution and thereafter drying the separated immobilized lipase to a water content of between about 2 and 40%.
A method for interesterification of fats which comprises passing solvent free, melted fat(s) through a column of immobilized Mucor miehei lipase preparation at a temperature in the range of 50.degree. C.-85.degree. C., said immobilized Mucor miehei lipase preparation being a particulate macroporous weak anion exchange resin having particle size such that more than 90% of the resin particles have a size between 100-1000 .mu.m whereon is bound Mucor miehei lipase, said Mucor miehei immobilized lipase preparation being of 2-20% water content when starting to pass the melted fat through the column of immobilized lipase.
Dependencies are inferred from the claim text.