Vectors for transformation by ascomycetes
AssigneeThe Regents of the University of California
InventorsWilliam E. Timberlake, John E. Hamer, M. Melanie Yelton
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Abstract
Vectors and procedures are provided that enable genetic manipulation of the filamentous ascomycetes such as Aspergillus nidulans and Aspergillus niger. The systems of the invention permit transformation of various Aspergillus strains as well as the production and secretion of desired foreign proteins. Also provided are cosmid vectors which enable the isolation, cloning, sequencing and modifications of genes from the filamentous ascomycetes.
Claims23
A vector effective in transformation of a bacterial or filamentous ascomycete host which vector comprises:
- (a) a DNA sequence comprising the Aspergillus trpC gene;
- and
- (b) replication and marker sequences from a pBR329 or derivatives thereof.
A cosmid vector or a derivative thereof which comprises:
- (a) a DNA sequence comprising the Aspergillus trpC gene,
- (b) replication and marker sequences operable in a bacterial host;
- and
- (c) a unique restriction cleavage site external to the gene of (a).
Dependencies are inferred from the claim text.