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US 4,814,268 AGranted
Methods for propagating fastidious human viruses and for producing purified suspensions thereof
OwnerJohn W. Kreider, Mary K. Howettinventors
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Abstract
Pr U.S. GOVERNMENT RIGHTS The invention described herein was made in the course of work under a grant or award from the National Institutes of Health. The U.S. Government has certain rights in this invention pursuant to such grant or award.
Claims27
A method for propagating a fastidious human virus comprising the steps of:
- extracting a fastidious human virus selected from the group consisting of human papillomavirus, varicella-zoster virus, cytomegalovirus, and gastroenteritis virus from material containing said virus;
- infecting human tissue known to be susceptible to said virus with said extracted virus;
- placing said infected tissue beneath the renal capsule of an immuno-compromised animal;
- allowing said infected tissue to remain in said immuno-compromised animal until recoverable quantities of said virus are produced;
- and
- recovering said virus from said tissue.
3 more elements
A method for propagating human papillomavirus (HPV) comprising the steps of:
- extracting HPV from human vulvar condylomata acuminata that contains said HPV;
- infecting human foreskin with said HPV by incubating said extracted HPV with said foreskin;
- placing said infected foreskin beneath the renal capsule of an athymic mouse;
- allowing said infected foreskin to remain in said mouse until recoverable quantities of HPV are produced;
- and
- recovering said HPV from said infected foreskin.
3 more elements
A purified viral suspension of HPV-11.
A method for preparing a vaccine against a fastidious human virus comprising the steps of:
- extracting a fastidious human virus selected from the group consisting of human papillomavirus, varicella-zoster virus, cytomegalovirus, and gastroenteritis virus from material containing said virus;
- infecting human tissue known to be susceptible to said virus with said extracted virus;
- placing said infected tissue beneath the renal capsule of an immuno-compromised animal;
- allowing said infected tissue to remain in said immuno-compromised animal until recoverable quantities of said virus are produced;
- recovering said virus from said tissue;
- purifying said virus;
- attenuating or killing said virus;
- and
- adding an immunologically effective amount of said attenuated or killed virus to a pharmacologically acceptable carrier.
6 more elements
Dependencies are inferred from the claim text.