# US 4,810,634 A · Pseudorabies virus mutants incapable of producing glycoprotein X

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 4,810,634 A
- Document ID: US4810634A
- Status: Granted
- Kind: A
- Application number: 07/049,865
- Filed: 1987-03-27
- Granted: 1989-03-07
- Earliest claimed: 1985-07-29
- Assignees: The Upjohn Company
- Inventors: Leonard E. Post; Darrell R. Thomsen
- Web page: https://patentgrep.com/patent/US4810634A

## CPC

- A61K 39/245: Herpetoviridae, e.g. herpes simplex virus
- A61K 39/12: Viral antigens
- A61P 31/12: Antivirals
- A61P 31/22: for herpes viruses
- C07K 14/005: from viruses
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 15/00: Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00)
- G01N 33/56983: {Viruses}
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- A61K 2039/5252: {inactivated (killed)}
- A61K 2039/552: {Veterinary vaccine}
- A61K 2039/70: {Multivalent vaccine}
- C12N 2710/16721: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2710/16722: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2710/16734: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y10S 530/826: Viruses
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Provided are a virus vaccine, comprising a properly incapacitated virus lacking an antigen of the wild-type virus which is useful for serologically distinguishing between vaccinated and infected animals, methods for distinguishing between vaccinated and infected animals and multivalent vaccines.

## Claims

### Claim 1

A non-naturally occurring pseudorabies virus, characterized in that upon replication it produces no glycoprotein X (gX) as a result of the viral DNA being altered or deleted.

### Claim 2 (depends on 1)

A pseudorabies virus according to claim 1, wherein said virus produces no gX as a result of a deletion, an insertion or both a deletion and an insertion in the gX gene.

### Claim 3 (depends on 2)

A pseudorabies virus according to claim 2, having a deletion in the gX gene.

### Claim 4 (depends on 2)

A pseudorabies virus according to claim 2, having an insertion in the gX gene.

### Claim 5 (depends on 1)

A pseudorabies virus according to claim 1, wherein the virus produces no functional thymidine kinase.

### Claim 6 (depends on 2)

A pseudorabies virus according to claim 2, wherein the virus produces no functional thymidine kinase.

### Claim 7 (depends on 3)

A pseudorabies virus according to claim 3, wherein the virus produces no functional thymidine kinase.

### Claim 8 (depends on 4)

A pseudorabies virus according to claim 4, wherein the virus produces no functional thymidine kinase.