Immunogenic conjugates of non-toxic E. coli LT-B enterotoxin subunit and capsular polymers
AssigneePraxis Biologics, Inc.
InventorsPorter W. Anderson, John D. Clements
Life of this patent
Estimating the term…
Abstract
A conjugate, which is the reductive amination product of an immunogenic capsular polymer fragment, having a reducing end and derived from the capsular polymer of a bacterial pathogen, and the non-toxic polypeptide binding subunit of the heat-labile enterotoxin of Escherichia coli (LT-BNT). Also disclosed, are methods for the preparation of the conjugates and for the preparation of vaccines containing the conjugates which elicits an effective level of antibodies in humans. By administering an immunogenic amount of the conjugates, active immunization against systematic infection in young mammals caused by bacterial pathogens can be induced.
Claims10
A conjugate of a PRP polysaccharide fragment, having reducing terminal groups derived from the capsular polysaccharide of Haemophilus influenzae type b by selective acidic hydrolysis of a portion of the ribosyl ribitol linkages therein, and a bacterial binding subunit, which binding subunit is a non-toxic polypeptide, having one or more immunoreactive and antigenic determinants of an LT-B subunit of the heat-labile enterotoxin of Escherichia coli (LT-BNT).
Dependencies are inferred from the claim text.