Peptide-beta-lactamase conjugates for enzyme-linked immunoassays
AssigneesNew York Blood Center, Inc., California Institute of Technology
InventorsAlexander R. Neurath, Stephen B. H. Kent, Nathan Strick
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Abstract
A reagent for an ELISA determination of an antibody, the reagent comprising a peptide covalently linked to beta-lactamase. The reagent can be used in the following method to detect antibodies in a sample which involves a. contacting the sample with protein A linked to a solid support, b. incubating the sample-protein A linked to the solid support, c. washing the incubated sample-protein A linked to the solid support, d. contacting the washed sample-protein A with the reagent, e. incubating the sample-protein A and reagent, f. washing the incubated sample-protein A-reagent, and g. determining the enzymatic activity of the resultant mass.
Claims10
A reagent for an ELISA determination of antibodies specific for the pre-S region of the hepatitis B virus envelope protein, the reagent comprising a peptide covalently linked to beta-lactamase, wherein said peptide is a synthetic peptide having an amino acid sequence corresponding to a sequence of amino acids in the pre-S region of the hepatitis B virus envelope protein and wherein said peptide mimics an antigenic determinant of said protein.
A method for detecting a given antibody in a sample, said antibody being specific for the pre-S region of the hepatitis B virus the method comprising:
- a. contacting the sample with protein A on a solid support,
- b. incubating the resultant mass from step a,
- c. washing the resultant mass from step b,
- d. contacting the washed mass from step c with a peptide-beta-lactamase conjugate, said peptide of said conjugate mimicing an antigenic determinant of said protein, said peptide being a synthetic peptide having an amino acid sequence corresponding to a sequence of amino acids in the pre-S region of hepatitis B virus envelope protein,
- e. incubating the resultant mass from step d,
- f. washing the resultant mass from step e, and
- g. determining the enzymatic activity of the resultant mass from step f.
A reagent for an ELISA determination of antibodies specific for HTLV III/LAV, the reagent comprising a peptide covalently linked to beta-lactamase, wherein said peptide being a synthetic peptide having an amino acid sequence corresponding to a sequence of amino acids in HTLV III/LAV and wherein said peptide mimics an antigenic determinant of HTLV III/LAV.
A method for detecting a given antibody in a sample, said antibody being specific for HTLV III/LAV, the method comprising:
- a. contacting the sample with protein A on a solid support,
- b. incubating the resultant mass from step a,
- c. washing the resultant mass from step b,
- d. contacting the washed mass from step c with a peptide-beta-lactamase conjugate, said peptide of said conjugate mimicing an antigenic determinant of said protein, said peptide being a synthetic peptide having an amino acid sequence corresponding to a sequence of amino acids in HTLV III/LAV,
- e. incubating the resultant mass from step d,
- f. washing the resultant mass from step e, and
- g. determining the enzymatic activity of the resultant mass from step f.
Dependencies are inferred from the claim text.