PG gene and its use in plants
AssigneeCalgene, Inc.
InventorsWilliam R. Hiatt, Raymond E. Sheehy, Christine K. Shewmaker, Jean C. Kridl, Vic Knauf
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Abstract
Polygalacturonase DNA sequence and its use in modulating polygalacturonase expression in plant cells. DNA constructions are provided. The transit peptide finds use with heterologous peptides.
Claims14
A DNA sequence which is uninterrupted, encodes tomato polygalacturonase (PG) and is flanked by at least one non-wild type DNA sequence 5' to or 3' to said polygalacturonase encoding sequence.
A cDNA encoding for tomato polygalacturonase.
A DNA construct comprising a DNA sequence of at least 15 base pairs of a DNA sequence encoding tomato polygalacturonase (PG) joined, in the opposite orientation for expression, 5' to the 3' terminus of a transcriptional initiation region functional in plants.
A DNA construct comprising a transcriptional initiation region functional in plants joined at its 3' terminus to the 5' terminus of a tomato polygalacturonase transit peptide encoding sequence, said transit peptide encoding sequence joined to other than a sequence encoding mature tomato polygalacturonase.
A DNA construct comprising a transcription initiation region functional in a plant joined directly or through an intervening sequence to the 5' terminus of at least one of (a) at least 15 and no more than 259 nt of the 5'-terminal coding region of tomato polygalacturonase and (b) at least 39 nt of the 3'-terminal coding region of tomato polygalacturonase.
Dependencies are inferred from the claim text.