Water soluble zanthylium derivative substrates
AssigneeCoulter Electronics, Inc.
InventorsGary A. Mitchell, Gerald E. Jaffe, Marilyn M. Solorzano
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Abstract
Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation.
Claims13
A method to obtain a hydrolyzed product for determining the activity of a sample, said sample including at least a proteolytic enzyme;
- said sample may include one or more member of the group consisting of:
- an inhibitor, a cofactor, an activator, and an antiactivator, for said enzyme, said method comprising the steps of:
- admixing a xanthylium derivative substrate with said sample, and permitting the admixture to stand for a period of time sufficient to obtain a hydrolyzed product capable of being used for determining the activity in said sample;
- said xanthylium derivative substrate being selected from the group consisting of:
- ##STR3## and acid salts thereof, wherein:
- R.sub.1 is a water solubilizing radical selected from the group consisting of sacrosyl, pyroglutamyl, and glutaryl;
- R.sub.2 is xanthylium, 3',6'-diamino-9'-(2-carboxyphenyl);
- and
- R.sub.3 is xanthylium, 3'-amino-6'-hydroxy-9'-(2-carboxyphenyl).
Dependencies are inferred from the claim text.