Perfusate for the preservation of organs
AssigneeWisconsin Alumni Research Foundation
InventorsFolkert O. Belzer, James H. Southard
Life of this patent
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Abstract
The present invention relates to new compositions for the preservation of organs prior to implantation comprising hydroxyethyl starch substantially free of ethylene glycol, ethylene chlorohydrin and acetone in a pharmaceutically acceptable organ perfusate.
Claims8
A perfusate for the preservation of organs intended for implantation in a patient requiring such implantation, including:
- 5% hydroxyethyl starch having a molecular weight of from about 200,000 to about 300,000
- 25mM KH.sub.2 PO.sub.4
- 3mM glutathione
- 5mM adenosine
- 10mM glucose
- 10mM HEPES buffer
- 5mM magnesium gluconate
- 1.5mM CaCl.sub.2
- 105mM sodium gluconate
- 200,000 units penicillin
- 40 units insulin
- 16mg Dexamethasone
- 12mg Phenol Red
- pH 7.4-7.5
- wherein the hdroxyethyl starch is substantially free of ethylene glycol, ethylene chlorohydrin, sodium chloride and acetone;
- and the perfusate has an osmolality of about 320 mOSm/1.
A perfusate for the preservation of organs intended for implantation in a patient requiring such implantation including:
- a pharmacologically acceptable perfusate solution having an osmolality of about 320 mOSm/l;
- and
- 5 weight percent hydroxyethyl starch, wherein said starch is substantially free of ethylene glycol, ethylene chlorohydrin, sodium chloride and acetone and has a molecular weight of about 200,000 daltons.
A composition of matter comprising hydroxyethyl starch having a molecular weight of from about 150,000 to about 350,000 daltons, degree of substitution of from about 0.4 to about 0.7, and being substantially free of ethylene glycol, ethylene chlorohydrin, sodium chloride and acetone, and being substantially free of hydroxyethyl starch having a molecular weight of less than about 50,000 daltons.
A method for extending the preservation of organs intended for implantation in a patient requiring such implantation, said method comprising infusing said organ with a perfusate including:
- a pharmacologically acceptable perfusate solution having an osmolality of about 320 mOSm/l;
- and
- 5 weight percent hydroxyethyl starch, wherein said starch is substantially free of ethylene glycol, ethylene chlorhydrin, sodium chloride and acetone and has a molecular weight of about 200,000 daltons.
Dependencies are inferred from the claim text.