Back to results
US 4,798,792 AGranted
Bacterial hydroxylation of codeine
AssigneeE. I. Du Pont de Nemours and Company
InventorsPatricia A. Harder, Daniel A. Kunz
Life of this patent
Estimating the term…
Abstract
A microbial process for converting codeine to 14-hydroxycodeine is provided. This process comprises aerobically culturing codeine with bacteria of the genus Streptomyces for at least about 3 days in a rich medium such as soybean flour medium.
Claims15
A process for preparing 14-hydroxycodeine which comprises:
- contacting codeine or a water-soluble salt thereof with bacteria of the genus Streptomyces for a period of at least about three days while said bacteria are being aerobically cultured in a rich medium in which the growth nutrients are supplied in excess;
- and recovering 14-hydroxycodeine from the medium.
A process for preparing 14-hydroxycodeine which comprises:
- contacting codeine or a water-soluble salt thereof with Streptomyces griseus bacteria for a period of about 3 to 30 days while said bacteria are being aerobically cultured in a rich medium in which the growth nutrients are supplied in excess and selected from the group consisting of soybean flour medium, yeast malt-extract medium, and yeast malt-extract medium with L-tyrosine;
- the codeine being present in the rich medium at a concentration less than about 5mM;
- and recovering 14-hydroxycodeine from the medium.
A process for producing 14-hydroxycodeine which comprises:
- culturing a micro-organism belonging to the species Steptomyces griseus, Streptomyces griseolus, Streptomyces punipalus, or Streptomyces lincolnensis having the ability to produce said 14-hydroxycodeine in a nutrient medium in which the growth nutrients are supplied in excess and accumulating said 14-hydroxycodeine in said medium.
Dependencies are inferred from the claim text.