Solubilization of protein aggregates
AssigneeBunge (Australia) Pty. Ltd.
InventorsJoseph J. Patroni, Malcolm R. Brandon
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Abstract
A method for the recovery of proteins which method includes providing a source of protein in an insoluble form, a source of at least one cationic surfactant; treating the insoluble protein with the at least one cationic surfactant in an amount sufficient to effect solubilization without substantial modification to the structural backbone of the protein.
Claims20
A method for the recovery of proteins which method includes providing a source of protein in an insoluble form, a source of at least one cationic surfactant;
- treating the insoluble protein with the at least one cationic surfactant, in the absence of denaturing guanidine hydrochloride and urea denaturing agents, in an amount sufficient to effect solubilisation without substantial modification to the structural backbone of the protein.
A method for the recovery of proteins which method includes providing a source of proteins in an insoluble form, a source of at least one cationic surfactant, and a source of at least one polar organic solvent;
- treating the insoluble protein with a mixture of from approximately 5 to 70% volume/volume of the at least one polar organic solvent and at least one cationic surfactant in an amount and sufficient to effect solubilisation without substantial modification to the structural backbone of the protein, in the absence of denaturing guanidine hydrochloride and urea denaturing agents;
- and separating the solubilised protein from the resulting solution.
Dependencies are inferred from the claim text.