Magnetic-polymer particles
AssigneeImmunicon Corporation
InventorsCharles S. Owen, John C. Silvia, Louis D'Angelo, Paul A. Liberti
Life of this patent
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Abstract
A magnetic-polymer particle, useful in immunoassay techniques and various other biological/medical applications is produced by coprecipitation of transition metals in the presence of a polymer having available coordination sites. These particles are capable of forming stable aqueous suspensions and may be easily resuspended after agglomeration.
Claims44
A process for the preparation of magnetic-polymer particles comprising the steps of:
- (a) combining a first aqueous solution of at least two species of transition metal ions capable of reacting with each other to form a magnetic precipitate and a polymer having available coordination sites in proportions adapted to produce a resuspendable product;
- (b) reacting said transition metal ions in the presence of said polymer to form a magnetic precipitate comprising magnetic-polymer particles;
- (c) recovering said magnetic-polymer particles.
A resuspendable magnetic-polymer particle comprising a particulate, magnetic reaction product of a water-soluble form of iron and a water-soluble polymer having available coordination sites.
A process for the preparation of ferromagnetic-polymer particles comprising the steps of:
- (a) preparing an aqueous solution of iron capable of reacting to form a magnetic precipitate and a polymer having available coordination sites;
- (b) adding a stoichiometric excess of a strong base;
- (c) agitating said solution;
- (d) allowing the magnetic-polymer particles to precipitate;
- (e) recovering said magnetic-polymer particles.
A process for the assay of a predetermined species comprising the steps of:
- (a) preparing an aqueous solution of transition metal ions capable of reacting to form a magnetic precipitate and a polymer having available coordinate sites in proportions adapted to produce resuspendable particles;
- (b) reacting said metal ions in the presence of said polymer to form a magnetic precipitate comprising magnetic-polymer particles;
- (c) recovering said magnetic-polymer particles;
- (d) resuspending said magnetic-polymer particles in an aqueous solution;
- (e) reacting said magnetic-polymer particles with both a bifunctional compound adapted to form primarily extra-particulate bonds, and a biofunctional ligand, said biofunctional ligand being specific in its ability to bond with said predetermined species;
- (f) exposing a mixture containing an unknown quantity of said predetermined species to a suspension of said magnetic-polymer particles containing said biofunctional ligand, thereby causing said biofunctional ligand to bond to said predetermined species;
- (g) passing said mixture through a magnetic filter having a magnetic field, said filter adapted to retain said magnetic-polymer particles;
- (h) removing said magnetic field from said filter and eluting said retained magnetic-polymer particles;
- (i) analyzing said eluted magnetic-polymer particles to provide desired data relative to said predetermined species.
A process for the preparation of magnetic-polymer particles comprising the steps of:
- (a) combining a first aqueous solution comprising at least two species of transition metal ions capable of reacting with each other to form a magnetic precipitate, and a polymer having biofunctional activity and having available coordination sites in proportions adapted to produce a resuspendable precipitate product having biofunctional activity;
- (b) reacting said transition metal ions in the presence of said polymer to form a magnetic precipitate comprising a complex of said metal and said biofunctional polymer;
- (c) recovering said magnetic precipitate from said first solution.
Dependencies are inferred from the claim text.