Immunoassay of free kappa light chains for the detection of multiple sclerosis
AssigneeUniversity of Rochester
InventorsRichard A. Rudick, Robert M. Herndon, Jean M. Bidlack
Life of this patent
Estimating the term…
Abstract
Multiple sclerosis (MS) is diagnosed by determining with a quantitative immunoassay the quantity of free kappa light chains in the cerebrospinal fluid of a patient. The immunoassay is preferably a radioimmunoassay. In carrying out the immunoassay, the free kappa light chains are combined with antiserum specific thereto. This method of diagnosing MS has high sensitivity in distinguishing between normal patients and patients affected with MS.
Claims7
In the diagnosis of multiple sclerosis by detecting components of the cerebrospinal fluid of a patient, the improvement which comprises directly determining by a quantitative immunoassay the quantity of free kappa light chains in the cerebrospinal fluid by combining the free kappa light chains in the fluid with antiserum specific thereto, and from the quantity determined diagnosing whether said patient has multiple Sclerosis.
A method for the detection of multiple sclerosis by a quantitative radioimmunoassay which comprises the steps of:
- 1-combining separately a plurality of different amounts of free kappa light chains with an antibody specific therefor and with radiolabeled free kappa light chains,
- 2-measuring the radioactivity present in said combinations,
- 3-combining cerebral spinal fluid of a patient with said antibody and said radiolabeled free kappa light chains,
- 4- measuring the radioactivity of the combination formed in step 3 and
- 5-comparing the radioactivity level of step 2 with that of step 4 to determine if said patient has multiple sclerosis.
A method for the detection of multiple sclerosis by a quantitative radioimmunoassay, which comprises the steps of:
- 1-isolating free kappa light chains from a source,
- 2-isolating an antiserum specific for free kappa light chains from another source,
- 3-radiolabeling a portion of said free kappa light chains,
- 4-combining an amount of said free kappa light chains with optimal amounts of said antiserum and said radiolabeled free kappa light chains,
- 5-allowing said combination sufficient time to react and form a precipitate, and 6-measuring the radioactivity of said precipitate;
- 7-repeating steps 4-6 with a plurality of different amounts of free kappa light chains to produce a standard curve;
- 8-repeating steps 4-6 with a test sample substituted for said free kappa light chains in step 4, wherein said test sample comprises cerebral spinal fluid isolated from a patient;
- and
- 9-comparing the result of step 8 with the standard curve set forth in step 7 as an indication of the quantity of free kappa light chains in said patient and determining from said quantity of free kappa light chains whether said patient has multiple sclerosis.
Dependencies are inferred from the claim text.