Back to results
US 4,791,067 AGranted
Agglutination immunoassay for hapten involving monoclonal antibody of IgA class reagent
AssigneeFisher Scientific Company
InventorsMark I. Sheiman, Kwok K. Yeung, Teresa H. Chan
Life of this patent
Estimating the term…
Abstract
An assay method and kit for a hapten such as theophylline employs a first, latex reagent and a second, antibody reagent. By using a monoclonal antibody of the IgA class in the second reagent, interference by patient rheumatoid factor is prevented.
Claims14
A method for detecting a hapten in a serum plasma or blood sample which comprises the steps:
- (a) Admixing the sample with:
- (1) a first reagent comprising particles bearing the hapten or an analog of the hapten suspended in an aqueous medium, and
- (2) a second reagent comprising an antibody against the hapten in an amount and of an activity sufficient to agglutinate the particles in the absence of hapten in the sample but to cause reduced amounts of agglutination with increasing amounts of hapten in the sample;
- and
- (b) detecting the degree of agglutination as a measure of the presence and amount of hapten in the sample;
- characterized by the antibody in the second reagent being a monoclonal antibody of the IgA class, whereby interference by rheumatoid factor or Clq in the sample is eliminated.
3 more elements
A kit for detecting a hapten in a serum plasma or blood sample which comprises:
- (a) A first reagent comprising particles bearing the hapten or an analog of the hapten suspended in an aqueous medium and
- (b) A second reagent comprising a monoclonal antibody against the hapten of the IgA class of activity capable of agglutinating the particles of the first reagent.
Dependencies are inferred from the claim text.