FVR vaccine
AssigneeDiamond Scientific Co.
InventorsGary P. Wiesehahn, Richard E. Giles, David R. Stevens
Life of this patent
Estimating the term…
Abstract
Novel vaccines for feline viral rhinotracheitis are prepared by psoralen inactivation of live Feline Herpesvirus I by exposure to ultraviolet radiation in the presence of an inactivating furocoumarin. The resulting inactivated viruses are suitable as the immunogenic substances in vaccines, which vaccines are useful for inoculation of hosts susceptible to feline virus rhinotracheitis.
Claims10
A method of making a vaccine useful for inoculation of a feline host susceptible to feline virus rhinotracheitis, said method comprising:
- (1) inactivating at least one feline Herpesvirus I isolate by
- (a) adding to said feline Herpesvirus I isolate a small but inactivating effective amount of a furocoumarin;
- and thereafter
- (b) exposing said feline Herpesvirus I to ultraviolet light having a wavelength within the range of from about 300 nm to 400 nm and an intensity of from about 0.1 mW/cm.sup.2 to 5 W/cm.sub.2 at a temperature below about 40.degree. C. for a time sufficient to render said virus noninfectious without destroying the characteristic immunologenic response of said feline Herpesvirus I isolate.
A method of preparing a vaccine useful for inoculation of a feline host susceptible to feline virus rhinotracheitis, said method comprising:
- subjecting at least one inactivated feline Herpesvirus I isolate to ultraviolet light having a wavelength within the range of from about 300 nm to 400 nm and an intensity of from about 0.1 mW/cm.sup.2 to 5W/cm.sup.2 at a temperature below about 40.degree. C. in the presence of an inactivating furocoumarin for a time sufficient to render said virus noninfectious without destroying its chacteristic immunogenic response.
Dependencies are inferred from the claim text.